Diagnostics for screening the blood supply

In vitro diagnostics for screening the blood provide: the brand new European regulation for IVD and the WHO IVD prequalification programme 

Blood transfusion stays a routine life-saving medical process that helps change blood misplaced as a result of surgical procedure, damage or illness. The standard of transfused blood is essential on this course of as blood donors should be freed from transfusion-transmissible infections and donated blood ought to be appropriate to that of the recipient. The standard of donated blood may very well be affected by the standard of in vitro diagnostic medical gadgets (IVDs) used within the screening course of.

Consequently, the necessity for high-quality, protected and well-performing IVDs to be used in transfusion drugs arises, accompanied by the necessity for tight laws on this area. Within the European Union, the brand new IVD Regulation will change the present IVD Directive inside a five-year transitional interval. Producers of IVDs are anticipated to totally adjust to the brand new Regulation by 26 Might 2022.

On this assessment, we tackle the foremost variations regarding advertising and marketing authorization and testing between this new Regulation and its predecessor. We additional current the primary components of the prequalification evaluation launched by the WHO for IVDs, together with disease-specific IVDs for blood screening laboratories.

Neuron Specific Enolase Protein
abx060902-100ug	Abbexa	100 ug	EUR 1387.2

Neuron Specific Enolase Antibody
F48200-0.4ML	NSJ Bioreagents	0.4 ml	EUR 379

Neuron Specific Enolase (NSE)/ENO2
CH23003	Neuromics	100 ul	EUR 214.8

Enolase, Neuron Specific (NSE) Antibody
20-abx102347	Abbexa	EUR 477.60 EUR 159.60 EUR 1328.40 EUR 644.40 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody
20-abx102348	Abbexa	EUR 477.60 EUR 159.60 EUR 1328.40 EUR 644.40 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody
20-abx102349	Abbexa	EUR 493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody
20-abx102350	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody
20-abx109806	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody
20-abx109807	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx159487	Abbexa	EUR 326.40 EUR 276.00	100 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody
20-abx172246	Abbexa	EUR 1011.60 EUR 526.80	1 mg 200 ug

Enolase, Neuron Specific (NSE) Antibody
20-abx172247	Abbexa	EUR 1011.60 EUR 526.80	1 mg 200 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx131861	Abbexa	EUR 376.80 EUR 944.40 EUR 493.20 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx131862	Abbexa	EUR 360.00 EUR 861.60 EUR 427.20 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx125813	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Neuron-Specific Enolase (ENO2) Antibody
20-abx126897	Abbexa	EUR 493.20 EUR 710.40	100 ul 200 ul

Enolase, Neuron Specific (ENO2) Antibody
20-abx112301	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Enolase, Neuron Specific (NSE) Antibody
20-abx129524	Abbexa	EUR 493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx002239	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Neuron Specific Enolase (NSE) Antibody
abx020636-1mg	Abbexa	1 mg	EUR 994.8

Neuron Specific Enolase (NSE) Antibody
abx020637-1mg	Abbexa	1 mg	EUR 994.8

Neuron Specific Enolase (NSE) Antibody
abx020638-1mg	Abbexa	1 mg	EUR 994.8

Neuron Specific Enolase (NSE) Antibody
abx020639-1mg	Abbexa	1 mg	EUR 994.8

Neuron Specific Enolase (NSE) Antibody
abx020640-1mg	Abbexa	1 mg	EUR 994.8

Neuron Specific Enolase (NSE) Antibody
abx020641-1mg	Abbexa	1 mg	EUR 994.8

Neuron-Specific Enolase (ENO2) Antibody
abx012133-100ug	Abbexa	100 ug	EUR 493.2

Neuron-Specific Enolase (ENO2) Antibody
20-abx013157	Abbexa	EUR 376.80 EUR 117.60 EUR 477.60 EUR 594.00	100 ug 10 ug 200 ug 300 µg

Neuron-Specific Enolase (NSE) Antibody
abx037802-100ug	Abbexa	100 ug	EUR 469.2

Enolase, Neuron Specific (ENO2) Antibody
abx036140-100ug	Abbexa	100 ug	EUR 469.2

Neuron-Specific Enolase (NSE) Antibody
abx037440-100ug	Abbexa	100 ug	EUR 469.2

Neuron-Specific Enolase (ENO2) Antibody
abx031704-400ul	Abbexa	400 ul	EUR 627.6

Neuron-Specific Enolase (ENO2) Antibody
abx031704-80l	Abbexa	80 µl	EUR 343.2

Neuron-Specific Enolase (ENO2) Antibody
abx031705-400ul	Abbexa	400 ul	EUR 627.6

Neuron-Specific Enolase (ENO2) Antibody
abx031705-80l	Abbexa	80 µl	EUR 343.2

Neuron-Specific Enolase (ENO2) Antibody
20-abx210136	Abbexa	EUR 526.80 EUR 393.60	100 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
20-abx329234	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Neuron-Specific Enolase (ENO2) Antibody
abx331441-100ul	Abbexa	100 ul	EUR 510

Neuron-Specific Enolase (ENO2) Antibody
abx331504-100ul	Abbexa	100 ul	EUR 510

Enolase, Neuron Specific (NSE) Antibody
20-abx270247	Abbexa	EUR 560.40 EUR 644.40 EUR 326.40 EUR 978.00 EUR 427.20	100 tests 200 tests 25 tests 500 tests 50 tests

Neuron-Specific Enolase (NSE) Antibody
abx411075-02mg	Abbexa	0.2 mg	EUR 678

Neuron-Specific Enolase (NSE) Antibody
abx411512-02mg	Abbexa	0.2 mg	EUR 678

Neuron-Specific Enolase (ENO2) Antibody
20-abx241083	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Neuron-Specific Enolase (ENO2) Antibody
20-abx241084	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Neuron-Specific Enolase (NSE) Antibody
abx235865-100ug	Abbexa	100 ug	EUR 577.2

Neuron-Specific Enolase (NSE) Antibody
abx235866-100ug	Abbexa	100 ug	EUR 577.2

Neuron-Specific Enolase (NSE) Antibody
abx235867-100ug	Abbexa	100 ug	EUR 610.8

Neuron Specific Enolase (NSE)/ENO2
RA22110	Neuromics	100 ul	EUR 490.8

Recombinant Enolase, Neuron Specific (NSE)
4-RPA537Hu01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Enolase, Neuron Specific expressed in: E.coli

Recombinant Enolase, Neuron Specific (NSE)
4-RPA537Hu02	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Enolase, Neuron Specific expressed in: E.coli

Recombinant Enolase, Neuron Specific (NSE)
4-RPA537Mu01	Cloud-Clone	EUR 528.58 EUR 265.20 EUR 1652.16 EUR 630.72 EUR 1141.44 EUR 429.60 EUR 3950.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Enolase, Neuron Specific expressed in: E.coli

Recombinant Enolase, Neuron Specific (NSE)
4-RPA537Mu02	Cloud-Clone	EUR 528.58 EUR 265.20 EUR 1652.16 EUR 630.72 EUR 1141.44 EUR 429.60 EUR 3950.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Enolase, Neuron Specific expressed in: E.coli

Recombinant Enolase, Neuron Specific (NSE)
4-RPA537Ra01	Cloud-Clone	EUR 528.58 EUR 265.20 EUR 1652.16 EUR 630.72 EUR 1141.44 EUR 429.60 EUR 3950.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Enolase, Neuron Specific expressed in: E.coli

NSE Antibody / Neuron Specific Enolase
RQ4566	NSJ Bioreagents	100ug	EUR 419

NSE Antibody / Neuron Specific Enolase
RQ5267	NSJ Bioreagents	100ul	EUR 419

NSE Antibody / Neuron Specific Enolase
V7731-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V7731-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V7731SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V8361-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V8361-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V8361SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V7210-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V7210-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V7210SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Neuron Specific Enolase Antibody (NSE)
F48200-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

Neuron Specific Enolase Antibody (NSE)
F48201-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

NSE Antibody / Neuron Specific Enolase
F54038-0.1ML	NSJ Bioreagents	0.1 ml	EUR 379

NSE Antibody / Neuron Specific Enolase
F54039-0.1ML	NSJ Bioreagents	0.1 ml	EUR 379

Neuron Specific Enolase ELISA Kit
E4703-100	Biovision	each	EUR 705.6

NSE Antibody / Neuron Specific Enolase
V3398-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3398-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3398SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3400-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3400-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3400SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3498-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3498-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3498IHC-7ML	NSJ Bioreagents	7 ml	EUR 499
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase
V3498SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Human Neuron Specific Enolase ELISA kit
E01N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Neuron Specific Enolase ELISA kit
E01N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Neuron Specific Enolase ELISA kit
E01N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Enolase, Neuron Specific (NSE) Protein
20-abx066423	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Enolase, Neuron Specific (NSE) Protein
20-abx066426	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Neuron-Specific Enolase (NSE) Protein
abx670067-50ug	Abbexa	50 ug	EUR 360

Human Neuron Specific Enolase (NSE) ELISA
LF-EK0169	Abfrontier	1×96T	EUR 723.6

Human Recombinant Neuron-Specific Enolase (NSE)
6362-100	Biovision	each	EUR 548.4

Goat Neuron Specific Enolase ELISA kit
E06N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Neuron Specific Enolase ELISA kit
E06N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Neuron Specific Enolase ELISA kit
E06N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit
E08N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit
E08N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit
E08N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit
E02N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit
E02N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit
E02N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Neuron Specific Enolase ELISA kit
E03N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Neuron Specific Enolase ELISA kit
E03N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Neuron Specific Enolase ELISA kit
E03N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Neuron Specific Enolase ELISA kit
E04N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Neuron Specific Enolase ELISA kit
E04N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Neuron Specific Enolase ELISA kit
E04N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit
E07N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit
E07N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit
E07N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Enolase, Neuron Specific (ENO2) Antibody (HRP)
20-abx108279	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody (HRP)
20-abx108280	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Mouse Enolase, Neuron Specific (NSE) Protein
20-abx066424	Abbexa	EUR 744.00 EUR 326.40 EUR 2230.80 EUR 878.40 EUR 543.60	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Enolase, Neuron Specific (ENO2) Protein
20-abx066425	Abbexa	EUR 744.00 EUR 326.40 EUR 2230.80 EUR 878.40 EUR 543.60	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody (Biotin)
20-abx105440	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody (Biotin)
20-abx105441	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody (FITC)
20-abx106859	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody (FITC)
20-abx106860	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Mouse Enolase, Neuron Specific (NSE) Protein
20-abx167196	Abbexa	EUR 744.00 EUR 326.40 EUR 2230.80 EUR 878.40 EUR 543.60	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (ENO2) Antibody Pair
abx117504-1pair5x96wellplates	Abbexa	1 pair (5x96 well plates)	EUR 1212

Enolase, Neuron Specific (NSE) Antibody Pair
20-abx370057	Abbexa	EUR 1914.00 EUR 1228.80	10 × 96 tests 5 × 96 tests

Rabbit Enolase, Neuron Specific (NSE) Protein
20-abx653280	Abbexa	EUR 777.60 EUR 326.40 EUR 2397.60 EUR 927.60 EUR 560.40	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (Biotin)
20-abx273178	Abbexa	EUR 543.60 EUR 292.80 EUR 1545.60 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (FITC)
20-abx273791	Abbexa	EUR 543.60 EUR 292.80 EUR 1545.60 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (FITC)
20-abx274000	Abbexa	EUR 543.60 EUR 292.80 EUR 1579.20 EUR 760.80 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (FITC)
20-abx274136	Abbexa	EUR 560.40 EUR 292.80 EUR 1663.20 EUR 777.60 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (Biotin)
20-abx271998	Abbexa	EUR 510.00 EUR 276.00 EUR 1429.20 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (Biotin)
20-abx272702	Abbexa	EUR 510.00 EUR 276.00 EUR 1462.80 EUR 710.40 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Enolase, Neuron Specific (NSE) Antibody (APC)
20-abx176282	Abbexa	EUR 1412.40 EUR 693.60	1 mg 200 ug

Polyclonal Neuron Specific Enolase (NSE) Antibody
APR08718G	Leading Biology	0.1ml	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Neuron Specific Enolase (NSE) . This antibody is tested and proven to work in the following applications:

Polyclonal Neuron-Specific Enolase (NSE) Antibody
APR08719G	Leading Biology	0.1mg	EUR 633.6
Description: A polyclonal antibody raised in Chicken that recognizes and binds to Human Neuron-Specific Enolase (NSE) . This antibody is tested and proven to work in the following applications:

Anti-Neuron Specific Enolase (NSE) Antibody
M02930-2	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Neuron Specific Enolase (NSE) Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Rabbit Neuron- specific enolase ELISA Kit
ELA-E0537Rb	Lifescience Market	96 Tests	EUR 1113.6

Monkey Neuron Specific Enolase ELISA kit
E09N0025-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Neuron Specific Enolase ELISA kit
E09N0025-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Neuron Specific Enolase ELISA kit
E09N0025-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Anti-NSE (Neuron specific enolase) antibody
STJ120175	St John's Laboratory	100 µl	EUR 562.8

Neuron Specific Enolase Antibody (phospho-Y236)
F48585-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

Human Neuron-specific enolase, NSE ELISA Kit
CSB-E07961h-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human Neuron-specific enolase, NSE in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Neuron-specific enolase, NSE ELISA Kit
1-CSB-E07961h	Cusabio	EUR 843.60 EUR 5811.60 EUR 3084.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Neuron-specific enolase, NSE in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Neuron-specific enolase,NSE ELISA Kit
CN-03318H1	ChemNorm	96T	EUR 546

Human Neuron-specific enolase,NSE ELISA Kit
CN-03318H2	ChemNorm	48T	EUR 364.8

Human Enolase, Neuron Specific (NSE) ELISA Kit
DLR-NSE-Hu-48T	DL Develop	48T	EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit
DLR-NSE-Hu-96T	DL Develop	96T	EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Enolase, Neuron Specific (ENO2) ELISA Kit
20-abx152555	Abbexa	EUR 7970.40 EUR 4250.40 EUR 990.00	10 × 96 tests 5 × 96 tests 96 tests

Human Neuron-Specific Enolase (NSE) CLIA Kit
abx195569-96tests	Abbexa	96 tests	EUR 990

Human Enolase, Neuron Specific (ENO2) ELISA Kit
abx573286-96tests	Abbexa	96 tests	EUR 877.2

Human Enolase, Neuron Specific (ENO2) CLIA Kit
20-abx491609	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Human Enolase, Neuron Specific (ENO2) ELISA Kit
abx253091-96tests	Abbexa	96 tests	EUR 877.2

Human Neuron-specific enolase,NSE ELISA Kit
201-12-0938	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Neuron Specific Enolase (NSE) ELISA (4X96T)
LF-EK0170	Abfrontier	4×96T	EUR 2454

Human Neuron Specific Enolase (NSE) ELISA (10X96T)
LF-EK0171	Abfrontier	10×96T	EUR 5380.8

Human Neuron Specific Enolase (NSE) ELISA (20X96T)
LF-EK0172	Abfrontier	20×96T	EUR 9369.6

Human NSE(Neuron-specific Enolase) ELISA Kit
EH0370	FN Test	96T	EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 1.406 ng/ml

Human Neuron-specific enolase(NSE)ELISA Kit
GA-E0954HM-48T	GenAsia Biotech	48T	EUR 346.8

Human Neuron-specific enolase(NSE)ELISA Kit
GA-E0954HM-96T	GenAsia Biotech	96T	EUR 559.2

Human Enolase, Neuron Specific (NSE) ELISA Kit
RD-NSE-Hu-48Tests	Reddot Biotech	48 Tests	EUR 573.6

Human Enolase, Neuron Specific (NSE) ELISA Kit
RD-NSE-Hu-96Tests	Reddot Biotech	96 Tests	EUR 794.4

Human Neuron-specific enolase(NSE)ELISA Kit
QY-E01645	Qayee Biotechnology	96T	EUR 472.8

Human Enolase, Neuron Specific (NSE) ELISA Kit
RDR-NSE-Hu-48Tests	Reddot Biotech	48 Tests	EUR 600

Human Enolase, Neuron Specific (NSE) ELISA Kit
RDR-NSE-Hu-96Tests	Reddot Biotech	96 Tests	EUR 830.4

Human Enolase, Neuron Specific (NSE) ELISA Kit
SEA537Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit
SEA537Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit
SEA537Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit
SEA537Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit
4-SEA537Hu	Cloud-Clone	EUR 5188.80 EUR 2739.60 EUR 703.20	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Enolase, Neuron Specific ELISA Kit (NSE)
RK01966	Abclonal	96 Tests	EUR 625.2

Human Neuron-specific enolase,NSE ELISA Kit
YLA0722HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Neuron-specific enolase,NSE ELISA Kit
YLA0722HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Human brain neuron-specific enolase (NSE/gamma enolase) protein (purified)
NSE15-N-100	Alpha Diagnostics	100 ug	EUR 489.6

Mouse Anti Human Neuron Specific Enolase Monoclonal Antibody
CABT-50869MH	Creative Diagnostics	1 ml	EUR 1388.4

Simultaneous detection of a number of mRNAs and proteins in bovine IVD cells and tissue with single cell decision 

Targets: Interactions of cells with their neighbors and influences by the encircling extracellular matrix (ECM) is mirrored in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells relying on ECM signalling cues akin to these of the intervertebral disc (IVD), this data is obscured or misplaced when cells are pooled for the generally used transcript evaluation by quantitative PCR or RNA sequencing.

As an alternative, these cells require means to analyse RNA transcript and protein distribution at a single cell or subcellular stage to determine totally different cell varieties and features, with out eradicating them from their surrounding signalling cues.

Outcomes: We developed a easy, sequential protocol combining RNA is situ hybridisation (RISH) and immunohistochemistry (IHC) for the simultaneous evaluation of a number of transcripts alongside proteins. This enables one to characterize heterogeneous cell populations on the single cell stage within the pure cell atmosphere and signalling context, each in vivo and in vitro. This protocol is demonstrated on cells of the bovine IVD, for transcripts and proteins concerned in mechanotransduction, stemness and cell proliferation.

Conclusions: A easy, sequential protocol combining RISH and IHC is introduced that permits for simultaneous data on RNA transcripts and proteins to characterize cells inside a heterogeneous cell inhabitants and sophisticated signalling environments akin to these of the IVD.

The brand new IVD Regulation 2017/746: a case examine at a big college hospital laboratory in Belgium demonstrates the necessity for clarification on the levels of freedom laboratories have to make use of lab-developed exams to enhance affected person care 

• Targets The brand new European In Vitro Diagnostic (IVD) Regulation 2017/746 (IVDR) restricts using lab-developed exams (LDT) after 26th Might 2022. There are not any knowledge on the influence of the IVDR on laboratories within the European Union. Strategies Laboratory exams carried out in UZ Leuven have been divided in 4 teams: core laboratory, immunology, particular chemistry, and molecular microbiology testing.
• Every take a look at was categorised as Conformité Européenne (CE)-IVD, modified/off-label CE-IVD, business Analysis Use Solely (RUO) or LDT. Every matrix was thought of a separate take a look at. Outcomes We discovered that 97.6% of the greater than 11.5 million outcomes/yr have been generated with a CE-IVD technique. Of the 922 totally different laboratory exams, nevertheless, solely 41.8% have been CE-IVD, 10.8% modified/off-label CE-IVD, 0.3% RUO, and 47.1% LDT. Off-label CE-IVD was primarily used to check different matrices not coated by the declare of the producer (e.g., pleural or peritoneal fluid). LDTs have been primarily used for particular chemistry, stream cytometry, and molecular testing.
• Excluding stream cytometry, the primary causes for the use of 377 LDTs have been lack of a CE-IVD technique (71.9%), analytical necessities (14.3%), and the actual fact the LDT was in use earlier than CE-IVD accessible (11.9%). Conclusions Whereas the big majority of outcomes (97.6%) have been generated with a CE-IVD technique, solely 41.8% of laboratory exams have been CE-IVD. There’s at present no different in the marketplace for 71.5% of the 537 LDTs carried out in our laboratory which don’t fall inside the scope of the present IVD directive (IVDD). Compliance with the IVDR would require a serious funding of effort and time.

Lack of sensitivity of an IVD/CE-labeled equipment concentrating on the S gene for detection of SARS-CoV-2 

Targets: New molecular exams for SARS-CoV-2 are quickly launched in response to the COVID-19 pandemic. The intention of this examine was to guage the analytical and the scientific efficiency of the VIASURE SARS-CoV-2 S gene RT-PCR Package on the BD Max™ system and to check outcomes with these obtained with the cobas® SARS-CoV-2 take a look at on the cobas® 6800 system.

Strategies: For testing the analytical efficiency, reference materials was used. Scientific samples (n=101) obtained from sufferers with signs appropriate to COVID-19 have been studied. Oro- and nasopharyngeal swabs have been collected through the use of both ESwab™ or UTM™ assortment programs.

Outcomes: When the analytical efficiency was evaluated, the pattern containing the bottom SARS-CoV-2 focus examined damaging with the VIASURE take a look at whereas outcomes obtained with the cobas® take a look at have been discovered to be concordant with the outcomes anticipated. Six out of the 101 scientific samples (5.9%) confirmed an inhibition with the VIASURE take a look at. When analyzing the remaining 95 scientific samples, 27 have been discovered to be damaging with each assays. Of 68 samples constructive with the cobas® take a look at, the VIASURE take a look at missed 21 (30.9 %) samples. All of these 21 samples had proven Ct values ≥ 31 with the cobas® 6800 system. Not one of the samples examined constructive with the VIASURE take a look at and damaging with the cobas® take a look at.

Conclusions: The VIASURE take a look at was impaired by a scarcity of sensitivity and a comparatively excessive variety of invalid outcomes. When utilizing the VIASURE take a look at for routine testing, a big variety of COVID-19 constructive samples would have been missed.

Present standing of growth of methylation biomarkers for in vitro diagnostic IVD functions 

A big quantity of analysis clearly exhibits that disease-related methylation modifications can be utilized as biomarkers in any respect phases of scientific illness administration, together with threat evaluation and predisposition screening via early diagnostics to personalization of affected person care and monitoring of the relapse and continual illness. Thus disease-related methylation modifications are a horny supply of the biomarkers that may have vital influence on precision drugs. Nevertheless, the interpretation of the analysis findings in methylation biomarkers discipline to scientific follow is on the very least not passable. That’s primarily as a result of the proof generated in analysis research indicating the utility of the disease-related methylation change to predict scientific final result is in majority of the circumstances not enough to postulate the diagnostic use of the biomarker.

The analysis research must be adopted by well-designed and systematic investigations of scientific utility of the biomarker that produce knowledge of enough high quality to satisfy regulatory approval for the take a look at for use to make clinically legitimate resolution. On this assessment, we describe methylation-based IVD exams at present authorised for IVD use or on the superior phases of the event for the diagnostic use. For every of these exams, we analyze the applied sciences that the take a look at makes use of for methylation detection in addition to describe the forms of the scientific research that have been carried out to point out scientific validity of the take a look at and warrant regulatory approval. The examples reviewed right here ought to assist with planning of scientific investigations and supply of the scientific proof required for the regulatory approval of potential methylation biomarker primarily based IVD exams.